Optimization of an Ultrasound-Assisted Extraction Method for the Extraction of Gingerols and Shogaols from Ginger (Zingiber officinale)

The goal of this study is to optimize a UAE method for the extraction main bioactive compounds present in ginger rhizome (gingerols and shogaols). Ginger (Zingiber officinale) has considerable content compounds, particular gingerols shogaols, with interesting pharmacological activities such as anti-inflammatory, antioxidant, anti-cancer, antimicrobial properties, among others. isolation these requires an efficient process short times employment specific non-toxic solvents humans. In work, optimization ultrasound-assisted (UAE) pungent rhizome, i.e., been carried out. For purpose, Box–Behnken design (BBD) used experimental through response surface methodology (RSM). percentage ethanol solvent, temperature, amplitude, cycle ultrasounds, well sample-to-solvent ratio, were variables be studied. Thus, solvent was identified most influential factor. Once extracted, identification shogaols performed by ultra-high-performance liquid chromatography (UHPLC) coupled quadrupole-time-of-flight mass spectrometer (Q-ToF-MS), quantification UHPLC diode array detector (DAD) detector. Finally, optimized required only 10 min time, presenting good repeatability intermediate precision levels (<5%). applied extract from diverse sources, thereby demonstrating its applicability highlighting potential variations compound concentrations across different samples based on factors origin, growing conditions,